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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in several tumors, which results in chromosome instability, and thus might lead to tumorigenesis. This protein was identified to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in marketing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two added splice variants ended up reported but specific nucleotide sequence information is just not yet offered.
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Blocking peptides are peptides that bind especially towards the goal antibody and block antibody binding. These peptide usually incorporates the epitope identified through the antibody.
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This tutorial outlines more doable optimization of mobile and immunoassay parameters to be sure the best possible effects are...
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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the best possible outcomes are...
Antibodies bound to the blocking peptide now not bind to the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, you can see which staining is unique.
Concentrate on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterized by website a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which add into the temporal coordination of each mitotic occasion. Cyclin E sorts a complex with and capabilities for a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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